Journal: The ISME Journal

Article Title: Dynamic nitrogen fixation in an aerobic endophyte of Populus

doi: 10.1093/ismejo/wrad012

Figure Lengend Snippet: ( A ) Representative hue saturation intensity (HSI) ion image of a 15 N-labeled WPB culture. Hues represent 15 N atom% values of individual cells. Incorporation is heterogeneous in pure culture of WPB. ( B ) Histogram representing NanoSIMS analyses of three biological replicates of the in vitro 15 N 2 fixation experiment. Bar height represents number of WPB cells in each enrichment class for each replicate. NanoSIMS was used to produce individual 15 N atom% estimates from six separate images for each replicate. Cell numbers ranged from 440 to 682 individual cells and enrichments of 0.6–70.3 atom% between the three replicates.

Article Snippet: Vials were flushed with argon/oxygen (70/30) and then dosed with either 3.5 ml 15 N 2 dinitrogen gas (Sigma) that had been cleaned with hydrochloric acid, to achieve a final concentration of ~20% oxygen, or with 5 ml laboratory air, to approximate equal nitrogen availability.

Techniques: Labeling, In Vitro

Journal: The ISME Journal

Article Title: Dynamic nitrogen fixation in an aerobic endophyte of Populus

doi: 10.1093/ismejo/wrad012

Figure Lengend Snippet: Atom% 15 N enrichment of peptides from WPB cultures collected after 3 or 9 days of incubation with either 15 N 2 or 14 N 2 , grouped by KEGG pathway. Datapoints represent individual peptides. Numbers at the bottom indicate the number of peptides detected in each treatment and time point. Overall, peptides associated with each pathway were significantly enriched in 15 N 2 vs. 14 N 2 treatments, as well as between 3 and 9 days of incubation (significance of pairwise comparisons shown on plots from Wilcoxon rank sum tests with Benjamini–Hochberg corrections for multiple testing), although individual peptide enrichment values varied. The y -axis is truncated to better show inter-treatment comparisons. Note that some pathway names have been abbreviated for visualization (“Nicotinate and nicotinamide metabolism” to “Nicotinate and nicotinamide”; “Tropane, piperidine and pyridine alkaloid biosynthesis” to “Tropane, piperidine, pyridine”).

Article Snippet: Vials were flushed with argon/oxygen (70/30) and then dosed with either 3.5 ml 15 N 2 dinitrogen gas (Sigma) that had been cleaned with hydrochloric acid, to achieve a final concentration of ~20% oxygen, or with 5 ml laboratory air, to approximate equal nitrogen availability.

Techniques: Incubation

Journal: iScience

Article Title: Accelerating ammonia synthesis in a membraneless flow electrolyzer through coupling ambient dinitrogen oxidation and water splitting

doi: 10.1016/j.isci.2023.106407

Figure Lengend Snippet: Key resources table

Article Snippet: The energy efficiency was calculated by the following equation:. ϵ = Ǝ / n N H 3 Equation 4 . where Ǝ is the applied electric energy and n is the measured ammonia molar amount. . 15 N 2 electrolysis The labeled isotope experiment was performed using 15 N 2 gas (98 at% 15 N 2 , Sigma-Aldrich) for electrolysis.

Techniques: Recombinant, Diffusion-based Assay, Software, Spectrophotometry